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After that MEFs were washed with PBS 1× twice and they were incubated in the media containing 20 mM HEPES, 140 mM NaCl, 16.1 mM KCl, 5.1 mM MgSO 4 , 2.7 mM CaCl 2 , 1.2 mM l-carnitine, 2% FFA-free BSA, 0.8 mM palmitate, and 2 μCi [ 14 C]palmitate (PerkinElmer—NEC534050UC) with pH adjusted to 7.4

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Per the reported advisory committee recommendation described below, the committee subsequently voted to recommend BPC-157's inclusion, but that recommendation was advisory and nonbinding